Mouse Nanog Polyclonal
Cat No: RCAB002P-F
Lot: 20090601
Quantity: 100 uL (0.20 mg/ml)
Storage: Store at +4°C for short term use. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Immunogen
Mouse Nanog protein
Specificity
Cross-reacts with Human, Monkey, and Mouse
Tested applications
Western blot
Immunocytochemistry
Application notes
WB: Use at a dilution of 1/300 - 1/2000
Immunocytochemistry: Use at a dilution of 1/150 - 1/700
Relevance
Transcriptional factors, OCT3/4 (POU5F1) and STAT3 function as key regulators in maintaining pluripotency of stem cells. Thus, POU5F1 and STAT3 have been widely used as molecular markers of pluripotential stem cells. Pluripotential cell-specific Nanog gene is a newly identified homeodomain-bearing transcriptional factor. Importantly, Nanog is expressed specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Therefore, Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human.
Raised in
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified
Storage buffer
Phosphate buffered saline incl. 0.1% sodium azide
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Western Blotting
Embryonic Stem Cells (ESCs): HM1-29
Mouse Embryonic Fibroblasts (MEFs)
1st Antibody:
New a-Nanog (1/500)
a-bActin (1/3000)
2nd Antibody
HRP-a-Rabbit IgG (1/500)
Detection: ECL-Plus (GE Healthcare)
Immunohistochemistry
Embryonic Stem Cells (ESCs): HM1-30
Mouse Embryonic Fibroblasts (PEFs)
Fix: 4% PFA/ PBS; 15 min/ RT
Blocking: 3% BSA, 2% Skim Milk, 0.1% Triton-X100/ PBS; 4° C/ON
1st Antibody:
a-Oct4 (Santa cruz: sc-5279) ; 1/50
mouse Nanog antibody 1/1000; 4 degC/ ON
2nd Antibody:
Oct4: Alexa Fluor 546-Anti-Mouse IgG 1/500
Nanog: Alexa Fluor 488-Anti-Rabbit IgG 1/500
Mounting with Slowfade with DAPI
