ReproFF2: Feeder-Free & Weekend-Labor-Free Medium
ReproFF2
A New Feeder-Free Medium for human ES/iPS cells
Cat No: RCHEMD006
Volume: 500 ml
Storage: -20 degC
- Culture maintenance required only three days/week
- Halves the amount of media use
- Reduce the labor cost for the weekend
- Maintains stable undifferentiated state
- Maintains robust differentiation capability
ReproFF2 is a new, improved feeder-free medium for human ES/iPS cells. Unlike conventional culture media that requires changing on a daily basis, ReproFF2 needs culture maintenance only three days a week, with no compromise made to the quality of the cells. Change the medium on Monday and Wednesday, passage the cells on Friday. Consequently, the cost and amount of medium used are halved, feeder cells need not be prepared, and the culture need not be cared during the weekend.
ReproFF2 was developed in collaboration with leading stem cell scientists for trouble-free, reproducible maintenance of ES and iPS cells. ReproFF2 has been developed to reduce the number of steps in the workflow and is in a highly convenient ready-to-go formulation. Just warm up, add bFGF*, and ReproFF2 is ready.
*: not necessary for all the cell lines
Tests using ALP staining, immunostaining, FACS analysis and karyotyping have confirmed its steady performance in comparison to conventional culture media. ReproFF2 is serum-free.
We have introductory offers available for ReproFF2. Please contact us by email if interested.
Offer 1: Free Sample
(Availability differs depending on the region.
Ask the local branch or distributor for the detail)
One bottle of ReproFF2 (100mL) is available as a free sample. One bottle/lab only, first-come first-served basis.
Offer 2: 30% OFF price for the first three months
(Japan, USA & EU only)
ReproFF2 is available at 30% OFF price for the first three months of your purchase. The amount you can buy during this period is not limited. To take advantage of this offer, you must be willing to provide ReproCELL with feedback about your experience using ReproFF2, which may be used as an anonymous testimonial in our marketing material. This offer will end in March 2012.
* Now all the ReproCELL products are sold at the price in USD. Please choose "United States" at the top "Select Region" menu for the accurate price.
Cells cultured with ReproFF2 retain important characteristics of undifferentiated pluripotent cells.
1. Maintanance of undifferentiated human ES cells with ReproFF2 
Human ES cell line KhES-1 (established in Kyoto University) remained undifferentiated in feeder-free culture with ReproFF2, as confirmed by morphology (A), alkaline phosphatase staining (B) and Oct 3/4 expression (C). In collaboration with Kyoto University.
2. Maintenance of undifferentiated human iPS cells with ReproFF2
Human iPS cell line 201B7 (established in Kyoto University) was kept undifferentiated in feeder-free culture with ReproFF2, as confirmed by morphology (A) and alkaline phosphatase staining (B). These results looked comparable to those of feeder-dependently cultured iPS cells (C and D).
Human iPS cells cultured with ReproFF2 retained expression of pluripotency marker genes Oct 3/4, SSEA-4, TRA-1-60 and Nanog, similarly to cells cultured on feeder cells.
3. FACS analysis of pluripotency marker expression in iPS cells
Human iPS cells cultured with ReproFF2 showed profiles comparable to those of feeder-dependently cultured cells when analysed with FACS for pluripotency marker genes SSEA-4, TRA-1-60 and TRA-1-81.
4-1. Differentiation into Cardiomyocytes
Human iPS cells cultured with ReproFF2 (upper row) could differentiate into cardiomyocytes, as those cultured with feeder cells (lower row). The cell clumps were beating, and the morphology and the expression of the cardiomyocyte markers were confirmed as shown above.
4-2. Differentiation into Neurons
Human iPS cells cultured with ReproFF2 (upper row) could differentiate via Rosetta stage into dopaminergic neurons, as those cultured with feeder cells (lower row). The expression of betaIII-tubulin was observed in neurites, and expression of tyrosine hydroxylase (TH, the enzyme for dopamine biosynthesis) was observed. Dopamine release and uptake were also confirmed.
