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rh FGF-2 (146aa)

rh FGF-2 (146aa)

Human Recombinant FGF-2 (146 aa)

Cat.No.: RCHEOT002    25 ug
             RCHEOT003  250 ug

bFGF is a critical component of human embryonic and induced pluripotent stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state*. It is typically used at a concentration of 4-5 ng/ml for both feeder dependent and feeder free culture. ReproCELL bFGF is functionally tested to maintain pluripotency in stem cells.

*:not necessary for all the cell lines

 

 

* Now all the ReproCELL products are sold at the price in USD. Please choose "United States" at the top "Select Region" menu for the accurate price.


Molecular Mass The 146 amino acid residue recombinant protein has a molecular mass of approximately 16.0K
Concentration 1 mg/mL
Buffer 10 mM Tris-HCL, 1mM DTT, 2M NaCL, pH7.5
Filtered 0.22 μm filter
Purity over 97%, as determinded by SDS-PAGE and visualized by CBB stain
Activity The biologocal activity of recombinant human FGF basic was monitored in a mitogenic assay by WST8